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  • 1.  Suggestion on refurbished or cheaper qPCR taking 10ul

    Posted 5 days ago
    Hello everyone, 
    My lab has recently acquired a CytAssist for Visium assays, and we do not have a qPCR. We do have a ddPCR QX200. I am reaching out to know if any of you are aware of options for a cheaper model of qPCR machines (also refurbished), any ways to utilize ddPCR, or any other ways to determine the cycle number for library prep for the Visium assays.

    Any input will be greatly appreciated.

    Thank you in advance,

    Mrinmoyee Majumder, Ph.D.
    Hollings Cancer Center
    Medical University of South Carolina
    68 President Street, BE411
    Charleston, SC 29425
    843-876-2293

    "If you publish a manuscript including research supported by the Translational Science Lab Shared Resource, the Hollings Cancer Center recommends the following text to be placed in the acknowledgement section: "Supported in part by the Translational Science Lab Shared Resource, Hollings Cancer Center, Medical University of South Carolina (P30 CA138313)."





  • 2.  RE: Suggestion on refurbished or cheaper qPCR taking 10ul

    Posted 4 days ago

    Hi Mrinmoyee,

    My lab purchased a Q qPCR machine from Quantabio about 8 months ago and it has worked great for our minimal qPCR needs: https://www.quantabio.com/product/products-pcr-instrumentation-q/

    We requested a demo prior to purchase so we confirmed that it would work for us.  We use the Q to validate user-prepared libraries and determine the appropriate number of amplification cycles to perform when preparing a couple of library types.

    I hope this helps!

    Emily



    ------------------------------
    Emily Crisovan, M.Sc.
    Operations Manager, Genomics Core
    Michigan State University
    Research Technology Support Facility (RTSF)
    612 Wilson Rd | Plant Biology Room S-20 | East Lansing, MI 48824
    pankeyem@msu.edu
    ------------------------------



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