Heidi,
You may want to check with Dave Adams from the BRCF Flow Cytometry Core. They have a Tyto.
Claudia Lalancette, PhD
Managing Director Epigenomics Core
BRCF University of Michigan Medicine
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Claudia Lalancette
Managing Director
University of Michigan Health Systems
Ann Arbor MI
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Original Message:
Sent: 11-07-2023 11:09
From: Heidi Monroe
Subject: Levicell from Levitas Bio for live/dead cell separation
Do you know the lower limit of the Tyto? We run some very limited samples (under 20,000 cells) with no good solution for dead cell removal, and increase risk of clog on the 10X.
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Heidi Monroe
Managing Director
Single Cell Core
University of Pittsburgh
Original Message:
Sent: 11-07-2023 10:14
From: David Leclerc
Subject: Levicell from Levitas Bio for live/dead cell separation
I'm happy to be convinced otherwise, but I'm not sold on the Levicell right now. I'm unsure about the performance i.e. percentage of dead cell removal, cell loss, and range of samples it can handle. But my reservation is mostly with the fact that this is a one trick pony - I don't know that it does anything but remove dead cells. The minute another group comes in with a better dead cell removal solution, the Levicell box won't have a much of a purpose anymore.
We have a MACSQuant Tyto which has been working great the few times we were asked to clean up dead cells from a sample. The price tag is vastly different to purchase the instrument of course, but not so much once the box is in your lab.
Again, happy to be proven wrong on this one!
Cheers!
David Leclerc, Director
Cytometry and Antibody Technology Facility
University of Chicago
BSLC R020
924 E 57th St
Chicago, IL 60637
Office: 773.702.5582
Lab: 773.702.9212
Thank you for your support of the CAT Facility.
Please remember to reference us in your publications.
Original Message:
Sent: 11/6/2023 11:43:00 AM
From: Heidi Monroe
Subject: Levicell from Levitas Bio for live/dead cell separation
Does anyone use the Levicell for live/dead cell separation? We had a demo for a week and saw improvements in viability from 50%-80%. We have a high number of samples with low viability that just don't have enough cells to sort or use a bead based live/dead kit. I think the Levicell could rescue some of these samples that we often need to load at less than 50% viability.
What are other cores doing for live/dead separation on low input samples? Are there alternatives to the Levicell?
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Heidi Monroe
Managing Director
Single Cell Core
University of Pittsburgh
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