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  • 1.  Advice needed for automation of Sanger sequencing

    Posted 10-01-2023 03:20

    Hi everyone

     

    Does anyone have experience automating Sanger sequencing? Our complete workflow from reaction setup, cycle sequencing, to reaction cleanup is currently done manually. We process approx. 40,000 samples a year and would like to automate this to reduce handling errors, improve reliability, and minimize hands-on time.

     

    Best wishes

    Patrick Driguez

     

    Patrick Driguez PhD

    Team Lead/ Staff Scientist

    Sanger and Third Generation Sequencing

    4700 King Abdullah University of Science and Technology,

    Thuwal, 23955-6900, Kingdom of Saudi Arabia

    Mob: +966 56 727 0699  Office: +966 (12) 8082499

    patrick.driguez@kaust.edu.sa

    signature_3869454545

     

     



  • 2.  RE: Advice needed for automation of Sanger sequencing

    Posted 10-19-2023 13:25

    Hi Patrick, while I can't help with automating the actual set up of the cycle sequencing reactions (we type of the sample sheet and PCR off of that), we do use a Beckman robot and magnetic beads to clean up the cycle sequencing reactions in 96-well plates.  It is super fast, reliable and the robot does a much better job than we can by doing it manually.  Hope this helps.  Jodie



    ------------------------------
    Jodie Franklin
    Research Associate
    Johns Hopkins Univ/Sch of Med
    Baltimore MD
    ------------------------------



  • 3.  RE: Advice needed for automation of Sanger sequencing

    Posted 10-22-2023 01:55

    Hi Jodie, thanks for the info - it's very useful. We use XTerminator beads but magnetic bead cleanup could be a better options for us. What beads do you use? Potentially we have OpenTron and BioMek robots available to us. 

    Best

    Patrick



    ------------------------------
    Patrick Driguez
    Team Lead - Sanger and Third Generation Sequencing
    King Abdullah University of Science and Technology
    Thuwal
    ------------------------------

    Original Message


  • 4.  RE: Advice needed for automation of Sanger sequencing

    Posted 10-23-2023 10:27

    Hi Patrick, you can use ADS Sequencing Reaction Cleaning Beads from Edge Biosystems or D-Pure from Nimagen.  We have used both and they work the same.  Jodie



    ------------------------------
    Jodie Franklin
    Research Associate
    Johns Hopkins Univ/Sch of Med
    Baltimore MD
    ------------------------------

    Original Message


  • 5.  RE: Advice needed for automation of Sanger sequencing

    Posted 10-26-2023 01:26

    Thanks Jodie

    We will check them out. 

    Patrick



    ------------------------------
    Patrick Driguez
    Team Lead - Sanger and Third Generation Sequencing
    King Abdullah University of Science and Technology
    Thuwal
    ------------------------------

    Original Message


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