Thanks Jodie
We will check them out.
Patrick
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Patrick Driguez
Team Lead - Sanger and Third Generation Sequencing
King Abdullah University of Science and Technology
Thuwal
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Original Message:
Sent: 10-23-2023 10:26
From: Jodie Franklin
Subject: Advice needed for automation of Sanger sequencing
Hi Patrick, you can use ADS Sequencing Reaction Cleaning Beads from Edge Biosystems or D-Pure from Nimagen. We have used both and they work the same. Jodie
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Jodie Franklin
Research Associate
Johns Hopkins Univ/Sch of Med
Baltimore MD
Original Message:
Sent: 10-22-2023 01:54
From: Patrick Driguez
Subject: Advice needed for automation of Sanger sequencing
Hi Jodie, thanks for the info - it's very useful. We use XTerminator beads but magnetic bead cleanup could be a better options for us. What beads do you use? Potentially we have OpenTron and BioMek robots available to us.
Best
Patrick
------------------------------
Patrick Driguez
Team Lead - Sanger and Third Generation Sequencing
King Abdullah University of Science and Technology
Thuwal
Original Message:
Sent: 10-19-2023 13:24
From: Jodie Franklin
Subject: Advice needed for automation of Sanger sequencing
Hi Patrick, while I can't help with automating the actual set up of the cycle sequencing reactions (we type of the sample sheet and PCR off of that), we do use a Beckman robot and magnetic beads to clean up the cycle sequencing reactions in 96-well plates. It is super fast, reliable and the robot does a much better job than we can by doing it manually. Hope this helps. Jodie
------------------------------
Jodie Franklin
Research Associate
Johns Hopkins Univ/Sch of Med
Baltimore MD
Original Message:
Sent: 10-01-2023 03:19
From: Patrick Driguez
Subject: Advice needed for automation of Sanger sequencing
Hi everyone
Does anyone have experience automating Sanger sequencing? Our complete workflow from reaction setup, cycle sequencing, to reaction cleanup is currently done manually. We process approx. 40,000 samples a year and would like to automate this to reduce handling errors, improve reliability, and minimize hands-on time.
Best wishes
Patrick Driguez
Patrick Driguez PhD
Team Lead/ Staff Scientist
Sanger and Third Generation Sequencing
4700 King Abdullah University of Science and Technology,
Thuwal, 23955-6900, Kingdom of Saudi Arabia
Mob: +966 56 727 0699 Office: +966 (12) 8082499
patrick.driguez@kaust.edu.sa
