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  • 1.  KOD Extreme Enzyme needed for PacBio Ampli-Fi protocol

    Posted 05-16-2025 14:21
    Hello ABRF Community,
    The Whitehead Genome Core is planning to do the PacBio Ampli-Fi library prep, but we need a third party enzyme that is backordered until August. PacBio is actively trying to come up with alternatives, but in the meantime, I wondered if anybody might have an excess and be willing to part with a tube? Our intention would be to buy it, with a PO or similar. I'd also be interested in hearing if others have successfully used alternative enzymes with this kit. 
    The item is: KOD Xtreme Hot Start DNA polymerase (200U) (Millipore Sigma 71975-3).
     
    Thanks in advance!

    --
    Jennifer Love (she/her/hers)
    Technical Associate II, Core Supervisor
    Whitehead Institute Genome Technology Core
    455 Main St. Room 325, Cambridge MA 02142
    (617) 258-8803


  • 2.  RE: KOD Extreme Enzyme needed for PacBio Ampli-Fi protocol

    Posted 05-19-2025 13:06

    Hi Jen,

    We ordered some recently and I'm 99% sure it arrived. I'd be happy to aliquot some out for you. How much do you need?


    Claire



    ------------------------------
    Claire Hartmann
    Director of Bauer Core
    Harvard University
    Cambridge MA
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