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Do you know the lower limit of the Tyto? We run some very limited samples (under 20,000 cells) with no good solution for dead cell removal, and increase risk of clog on the 10X. ------------------------------ Heidi Monroe Managing Director Single Cell Core University of Pittsburgh --------------- ...

Live cell recovery was between 40-80%. We had higher recovery when we started with more cells (>500k). ------------------------------ Heidi Monroe Managing Director Single Cell Core University of Pittsburgh ------------------------------

Does anyone use the Levicell for live/dead cell separation? We had a demo for a week and saw improvements in viability from 50%-80%. We have a high number of samples with low viability that just don't have enough cells to sort or use a bead based live/dead kit. I think the Levicell could rescue some ...

How do you charge for computing time? We're currently charging 1/4 time (1hr charge for every 4hrs), I'm not sure where that decision came from. I'm curious to hear what others are doing, and if it's based on how many jobs can run at one time. ------------------------------ Heidi Monroe Managing Director ...

I manage a Single Cell Sequencing Core (all 10X Genomics for now) and we are struggling with a few aspects of setting fees for bioinformatics analysis. A little background-I started here a little over a year ago, and we have an hourly analysis rate that incorporates both analyst time and computing time. ...