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Are there any universities that are receiving proteomics reagent discounts because you have purchased equipment from Thermo and you are paying for service contracts? I'm being told that they are two separate divisions within thermo (SCs and reagents) and therefore no discounts are given on reagents. ...

Joshua, what is your inside rate for a Sanger reaction and who is your outside competition around Wisconsin? I'm just curious as we cannot compete with outside competition in the DC/MD/VA region. Thanks! ------------------------------ Jodie Franklin Research Associate Johns Hopkins Univ/Sch of Med ...

Hi Patrick, you can use ADS Sequencing Reaction Cleaning Beads from Edge Biosystems or D-Pure from Nimagen. We have used both and they work the same. Jodie ------------------------------ Jodie Franklin Research Associate Johns Hopkins Univ/Sch of Med Baltimore MD ------------------------------

Hi Patrick, while I can't help with automating the actual set up of the cycle sequencing reactions (we type of the sample sheet and PCR off of that), we do use a Beckman robot and magnetic beads to clean up the cycle sequencing reactions in 96-well plates. It is super fast, reliable and the robot does ...

Good morning. Johns Hopkins used to have a CORE showcase before COVID. We did it every year for maybe 5 years. Food was offered so that was an incentive to get people to come however in my case, I don't think we got any new users from the event. Maybe other CORES at the event were successful in getting ...

I would have definitely thought I had assembled the valves incorrectly except the flow was stopping when I put it to the load position before I even took the injector apart to change the rotor seal and stator face. I thought for sure one was stuck or something and that changing them would fix the problem...no ...

Hi Zdenek, I actually cannot push liquid into the loop in the load position and then I over pressure. I've looked at the injector schematics and in the inject position it is flowing from the pump into the loop and then out to the column utilizing 4 of the ports. When it is in the load position, it flows ...

good morning, I am using a Waters 1525 pump with a 7725i manual injector. When the injector is in "inject" mode, everything flows correctly. When we put it into "load" mode the flow stops and the pressure goes up until it faults. I have changed the rotor seal and the stator face and I still have the ...